Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1294047..1294695 Replicon chromosome
Accession NZ_CP017535
Organism Mannheimia haemolytica strain 1498

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG609_RS06690 Protein ID WP_006251221.1
Coordinates 1294519..1294695 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG609_RS06685 Protein ID WP_006251222.1
Coordinates 1294047..1294463 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG609_RS06645 1290385..1290819 + 435 WP_006251230.1 hypothetical protein -
BG609_RS06650 1290834..1291823 + 990 WP_006251229.1 encapsulin -
BG609_RS06655 1291835..1292050 + 216 WP_075281089.1 hypothetical protein -
BG609_RS06660 1292053..1292430 + 378 WP_006251227.1 hypothetical protein -
BG609_RS06665 1292430..1292774 + 345 WP_006251226.1 hypothetical protein -
BG609_RS06670 1292776..1293144 + 369 WP_006251225.1 hypothetical protein -
BG609_RS06675 1293141..1293521 + 381 WP_006251224.1 hypothetical protein -
BG609_RS06680 1293525..1294007 + 483 WP_006251223.1 phage tail protein -
BG609_RS06685 1294047..1294463 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG609_RS06690 1294519..1294695 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG609_RS06695 1294791..1295462 + 672 WP_006251220.1 hypothetical protein -
BG609_RS06700 1295477..1295710 - 234 WP_020824316.1 hypothetical protein -
BG609_RS06705 1295879..1296337 + 459 WP_006251218.1 hypothetical protein -
BG609_RS06715 1296882..1299377 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1292588..1469162 176574


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67280 WP_006251221.1 NZ_CP017535:c1294695-1294519 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67280 NZ_CP017535:c1294695-1294519 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67280 WP_006251222.1 NZ_CP017535:c1294463-1294047 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67280 NZ_CP017535:c1294463-1294047 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References