Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1953338..1953877 Replicon chromosome
Accession NZ_CP017534
Organism Mannheimia haemolytica strain 1503

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG587_RS10285 Protein ID WP_006247804.1
Coordinates 1953608..1953877 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG587_RS10280 Protein ID WP_006247803.1
Coordinates 1953338..1953604 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG587_RS10225 1948738..1949373 - 636 Protein_1990 transposase -
BG587_RS10235 1949474..1950184 - 711 Protein_1991 tyrosine-type recombinase/integrase -
BG587_RS10240 1950088..1950411 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG587_RS10245 1950492..1950659 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG587_RS10250 1950667..1951041 - 375 WP_006247798.1 hypothetical protein -
BG587_RS10255 1951191..1951418 + 228 Protein_1995 phage terminase large subunit family protein -
BG587_RS10260 1951443..1951838 + 396 WP_006247799.1 phage tail protein -
BG587_RS10265 1951870..1952511 + 642 WP_006247800.1 hypothetical protein -
BG587_RS10270 1952594..1952995 + 402 WP_006247801.1 hypothetical protein -
BG587_RS10275 1953040..1953270 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG587_RS10280 1953338..1953604 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG587_RS10285 1953608..1953877 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG587_RS10290 1953952..1954215 + 264 WP_006247805.1 hypothetical protein -
BG587_RS10295 1954275..1954985 + 711 WP_006253312.1 tape measure protein -
BG587_RS10300 1954963..1955253 + 291 Protein_2004 C40 family peptidase -
BG587_RS10305 1955243..1955962 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG587_RS10310 1956184..1956810 + 627 WP_006247807.1 tail assembly protein -
BG587_RS10315 1956859..1957470 + 612 WP_006253310.1 hypothetical protein -
BG587_RS10320 1957581..1958267 + 687 WP_006247808.1 hypothetical protein -
BG587_RS14905 1958277..1958435 + 159 WP_006247809.1 hypothetical protein -
BG587_RS10325 1958444..1958743 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1940725..1964931 24206
- flank IS/Tn - - 1948681..1949373 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67274 WP_006247804.1 NZ_CP017534:1953608-1953877 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67274 NZ_CP017534:1953608-1953877 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67274 WP_006247803.1 NZ_CP017534:1953338-1953604 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67274 NZ_CP017534:1953338-1953604 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References