Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1923189..1923728 Replicon chromosome
Accession NZ_CP017532
Organism Mannheimia haemolytica strain 1512

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG583_RS10065 Protein ID WP_006247804.1
Coordinates 1923459..1923728 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG583_RS10060 Protein ID WP_006247803.1
Coordinates 1923189..1923455 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG583_RS10005 1918589..1919224 - 636 Protein_1945 transposase -
BG583_RS10015 1919325..1920035 - 711 Protein_1946 tyrosine-type recombinase/integrase -
BG583_RS10020 1919939..1920262 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG583_RS10025 1920343..1920510 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG583_RS10030 1920518..1920892 - 375 WP_006247798.1 hypothetical protein -
BG583_RS10035 1921042..1921269 + 228 Protein_1950 phage terminase large subunit family protein -
BG583_RS10040 1921294..1921689 + 396 WP_006247799.1 phage tail protein -
BG583_RS10045 1921721..1922362 + 642 WP_006247800.1 hypothetical protein -
BG583_RS10050 1922445..1922846 + 402 WP_006247801.1 hypothetical protein -
BG583_RS10055 1922891..1923121 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG583_RS10060 1923189..1923455 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG583_RS10065 1923459..1923728 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG583_RS10070 1923803..1924066 + 264 WP_006247805.1 hypothetical protein -
BG583_RS10075 1924126..1924836 + 711 WP_006253312.1 tape measure protein -
BG583_RS10080 1924814..1925104 + 291 Protein_1959 C40 family peptidase -
BG583_RS10085 1925094..1925813 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG583_RS10090 1926035..1926661 + 627 WP_006247807.1 tail assembly protein -
BG583_RS10095 1926710..1927321 + 612 WP_006253310.1 hypothetical protein -
BG583_RS10100 1927432..1928118 + 687 WP_006247808.1 hypothetical protein -
BG583_RS14435 1928128..1928286 + 159 WP_006247809.1 hypothetical protein -
BG583_RS10105 1928295..1928594 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1910576..1934782 24206
- flank IS/Tn - - 1918532..1919224 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67258 WP_006247804.1 NZ_CP017532:1923459-1923728 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67258 NZ_CP017532:1923459-1923728 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67258 WP_006247803.1 NZ_CP017532:1923189-1923455 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67258 NZ_CP017532:1923189-1923455 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References