67252

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2201954..2202604 Replicon chromosome
Accession NZ_CP017531
Organism Mannheimia haemolytica strain 1514

Toxin (Protein)


Gene name vapC Uniprot ID A0A547EDJ6
Locus tag BG604_RS11290 Protein ID WP_006249411.1
Coordinates 2202215..2202604 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A547EDM1
Locus tag BG604_RS11285 Protein ID WP_006249412.1
Coordinates 2201954..2202214 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG604_RS11270 2197642..2201175 + 3534 WP_006253629.1 transcription-repair coupling factor -
BG604_RS11275 2201338..2201625 + 288 WP_006251764.1 BrnT family toxin -
BG604_RS11280 2201626..2201838 + 213 WP_006251765.1 BrnA antitoxin family protein -
BG604_RS11285 2201954..2202214 + 261 WP_006249412.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG604_RS11290 2202215..2202604 + 390 WP_006249411.1 type II toxin-antitoxin system VapC family toxin Toxin
BG604_RS11295 2202624..2203862 + 1239 WP_006253630.1 peptidase T -
BG604_RS11325 2204605..2205390 + 786 WP_006251768.1 hypothetical protein -
BG604_RS11330 2205464..2206324 + 861 WP_006250124.1 metal ABC transporter permease -
BG604_RS11335 2206317..2207126 + 810 WP_006250123.1 metal ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14633.91 Da        Isoelectric Point: 7.1041

>T67252 WP_006249411.1 NZ_CP017531:2202215-2202604 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T67252 NZ_CP017531:2202215-2202604 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGTGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.54 Da        Isoelectric Point: 4.8951

>AT67252 WP_006249412.1 NZ_CP017531:2201954-2202214 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT67252 NZ_CP017531:2201954-2202214 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAAAAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A547EDJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A547EDM1

References