Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2374215..2374865 Replicon chromosome
Accession NZ_CP017530
Organism Mannheimia haemolytica strain 1532

Toxin (Protein)


Gene name vapC Uniprot ID A0A547EDJ6
Locus tag BG599_RS12230 Protein ID WP_006249411.1
Coordinates 2374215..2374604 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A547EDM1
Locus tag BG599_RS12235 Protein ID WP_006249412.1
Coordinates 2374605..2374865 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG599_RS12175 2369486..2370295 - 810 WP_006250123.1 metal ABC transporter permease -
BG599_RS12180 2370288..2371148 - 861 WP_006250124.1 metal ABC transporter permease -
BG599_RS12185 2371222..2372007 - 786 WP_006251768.1 hypothetical protein -
BG599_RS12225 2372957..2374195 - 1239 WP_006253630.1 peptidase T -
BG599_RS12230 2374215..2374604 - 390 WP_006249411.1 type II toxin-antitoxin system VapC family toxin Toxin
BG599_RS12235 2374605..2374865 - 261 WP_006249412.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG599_RS12240 2374981..2375193 - 213 WP_006251765.1 BrnA antitoxin family protein -
BG599_RS12245 2375194..2375481 - 288 WP_006251764.1 BrnT family toxin -
BG599_RS12250 2375644..2379177 - 3534 WP_147036343.1 transcription-repair coupling factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14633.91 Da        Isoelectric Point: 7.1041

>T67245 WP_006249411.1 NZ_CP017530:c2374604-2374215 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T67245 NZ_CP017530:c2374604-2374215 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGTGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.54 Da        Isoelectric Point: 4.8951

>AT67245 WP_006249412.1 NZ_CP017530:c2374865-2374605 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT67245 NZ_CP017530:c2374865-2374605 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAAAAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A547EDJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A547EDM1

References