Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1304198..1304846 Replicon chromosome
Accession NZ_CP017530
Organism Mannheimia haemolytica strain 1532

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG599_RS06745 Protein ID WP_006251221.1
Coordinates 1304670..1304846 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG599_RS06740 Protein ID WP_006251222.1
Coordinates 1304198..1304614 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG599_RS06700 1300419..1300853 + 435 WP_006251230.1 hypothetical protein -
BG599_RS06705 1300868..1301857 + 990 WP_006251229.1 encapsulin -
BG599_RS06710 1301869..1302201 + 333 WP_147036295.1 hypothetical protein -
BG599_RS06715 1302204..1302581 + 378 WP_006251227.1 hypothetical protein -
BG599_RS06720 1302581..1302925 + 345 WP_006251226.1 hypothetical protein -
BG599_RS06725 1302927..1303295 + 369 WP_006251225.1 hypothetical protein -
BG599_RS06730 1303292..1303672 + 381 WP_147036296.1 hypothetical protein -
BG599_RS06735 1303676..1304158 + 483 WP_006251223.1 phage tail protein -
BG599_RS06740 1304198..1304614 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG599_RS06745 1304670..1304846 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG599_RS06750 1304942..1305613 + 672 WP_006251220.1 hypothetical protein -
BG599_RS06755 1305628..1305861 - 234 WP_020824316.1 hypothetical protein -
BG599_RS06760 1306030..1306488 + 459 WP_006251218.1 hypothetical protein -
BG599_RS06770 1307033..1309528 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1270845..1329053 58208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67243 WP_006251221.1 NZ_CP017530:c1304846-1304670 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67243 NZ_CP017530:c1304846-1304670 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67243 WP_006251222.1 NZ_CP017530:c1304614-1304198 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67243 NZ_CP017530:c1304614-1304198 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References