Detailed information of TA system TA067242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1257035..1257675
• Replicon: chromosome
• Accession: NZ_CP017530
• Organism: Mannheimia haemolytica strain 1532

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG599_RS06360
• Protein ID: WP_006250075.1
• Coordinates: 1257493..1257675 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG599_RS06355
• Protein ID: WP_006250074.1
• Coordinates: 1257035..1257454 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG599_RS06330	1252543..1253349	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
BG599_RS06335	1253404..1254810	+	1407	WP_006253513.1	YdgA family protein	-
BG599_RS06340	1254877..1255917	-	1041	WP_020910232.1	IS481 family transposase	-
BG599_RS06350	1256055..1256699	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG599_RS06355	1257035..1257454	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG599_RS06360	1257493..1257675	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG599_RS06365	1257807..1258292	-	486	WP_015484250.1	hypothetical protein	-
BG599_RS06370	1258297..1258677	-	381	WP_006248823.1	hypothetical protein	-
BG599_RS06375	1258752..1259435	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG599_RS06380	1259566..1259766	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG599_RS13630	1259787..1260854	+	1068	WP_020824319.1	hypothetical protein	-
BG599_RS13635	1260864..1261022	+	159	WP_006247809.1	hypothetical protein	-
BG599_RS06395	1261031..1261330	-	300	WP_006247810.1	XRE family transcriptional regulator	-
BG599_RS06400	1261302..1261691	-	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG599_RS06405	1261835..1262128	+	294	WP_006247812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1254877..1255917	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67242 WP_006250075.1 NZ_CP017530:c1257675-1257493 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67242 NZ_CP017530:c1257675-1257493 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67242 WP_006250074.1 NZ_CP017530:c1257454-1257035 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67242 NZ_CP017530:c1257454-1257035 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9