Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1082666..1083205 Replicon chromosome
Accession NZ_CP017530
Organism Mannheimia haemolytica strain 1532

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG599_RS05455 Protein ID WP_006247804.1
Coordinates 1082666..1082935 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG599_RS05460 Protein ID WP_006247803.1
Coordinates 1082939..1083205 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG599_RS05415 1077800..1078099 + 300 WP_006247810.1 XRE family transcriptional regulator -
BG599_RS13620 1078108..1078266 - 159 WP_006247809.1 hypothetical protein -
BG599_RS05420 1078276..1078962 - 687 WP_006247808.1 hypothetical protein -
BG599_RS05425 1079073..1079684 - 612 WP_006253310.1 hypothetical protein -
BG599_RS05430 1079733..1080359 - 627 WP_006247807.1 tail assembly protein -
BG599_RS05435 1080581..1081300 - 720 WP_006247806.1 preprotein translocase subunit YajC -
BG599_RS05440 1081290..1081580 - 291 Protein_1037 C40 family peptidase -
BG599_RS05445 1081558..1082268 - 711 WP_006253312.1 tape measure protein -
BG599_RS05450 1082328..1082591 - 264 WP_006247805.1 hypothetical protein -
BG599_RS05455 1082666..1082935 - 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG599_RS05460 1082939..1083205 - 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG599_RS05465 1083273..1083503 - 231 WP_006247802.1 DUF4035 domain-containing protein -
BG599_RS05470 1083548..1083949 - 402 WP_006247801.1 hypothetical protein -
BG599_RS05475 1084032..1084673 - 642 WP_006247800.1 hypothetical protein -
BG599_RS05480 1084705..1085100 - 396 WP_006247799.1 phage tail protein -
BG599_RS05485 1085125..1085352 - 228 Protein_1046 phage terminase large subunit family protein -
BG599_RS05490 1085502..1085876 + 375 WP_006247798.1 hypothetical protein -
BG599_RS05495 1085884..1086051 + 168 WP_006253314.1 DNA cytosine methyltransferase -
BG599_RS05500 1086132..1086455 + 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG599_RS05505 1086359..1087069 + 711 Protein_1050 tyrosine-type recombinase/integrase -
BG599_RS05515 1087170..1087805 + 636 Protein_1051 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1073038..1097112 24074
- flank IS/Tn - - 1087170..1087862 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67241 WP_006247804.1 NZ_CP017530:c1082935-1082666 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67241 NZ_CP017530:c1082935-1082666 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67241 WP_006247803.1 NZ_CP017530:c1083205-1082939 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67241 NZ_CP017530:c1083205-1082939 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References