Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1817800..1818440 Replicon chromosome
Accession NZ_CP017529
Organism Mannheimia haemolytica strain 1560

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG600_RS09365 Protein ID WP_006250075.1
Coordinates 1818258..1818440 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag BG600_RS09360 Protein ID WP_006250074.1
Coordinates 1817800..1818219 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG600_RS09335 1813347..1814153 + 807 WP_006248821.1 tryptophan synthase subunit alpha -
BG600_RS09340 1814203..1815609 + 1407 WP_006253513.1 YdgA family protein -
BG600_RS09345 1815676..1816682 - 1007 Protein_1817 IS481 family transposase -
BG600_RS09355 1816820..1817464 - 645 WP_006253274.1 tyrosine-type recombinase/integrase -
BG600_RS09360 1817800..1818219 - 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG600_RS09365 1818258..1818440 - 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG600_RS09370 1818572..1819057 - 486 WP_015484250.1 hypothetical protein -
BG600_RS09375 1819062..1819442 - 381 WP_006248823.1 hypothetical protein -
BG600_RS09380 1819517..1820200 - 684 WP_006248824.1 helix-turn-helix transcriptional regulator -
BG600_RS09385 1820331..1820531 + 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG600_RS13830 1820552..1821619 + 1068 WP_006253284.1 hypothetical protein -
BG600_RS13835 1821629..1821787 + 159 WP_006247809.1 hypothetical protein -
BG600_RS09400 1821796..1822095 - 300 WP_006247810.1 XRE family transcriptional regulator -
BG600_RS09405 1822067..1822456 - 390 WP_006247811.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG600_RS09410 1822600..1822893 + 294 WP_006250050.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67234 WP_006250075.1 NZ_CP017529:c1818440-1818258 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67234 NZ_CP017529:c1818440-1818258 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT67234 WP_006250074.1 NZ_CP017529:c1818219-1817800 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT67234 NZ_CP017529:c1818219-1817800 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References