Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1649749..1650389 Replicon chromosome
Accession NZ_CP017528
Organism Mannheimia haemolytica strain 1561

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG579_RS08525 Protein ID WP_006250075.1
Coordinates 1649749..1649931 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag BG579_RS08530 Protein ID WP_006250074.1
Coordinates 1649970..1650389 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG579_RS08480 1645296..1645589 - 294 WP_006247812.1 hypothetical protein -
BG579_RS08485 1645733..1646122 + 390 WP_015484247.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG579_RS08490 1646094..1646393 + 300 WP_006247810.1 XRE family transcriptional regulator -
BG579_RS13975 1646402..1646560 - 159 WP_006247809.1 hypothetical protein -
BG579_RS13980 1646570..1647637 - 1068 WP_006253284.1 hypothetical protein -
BG579_RS08505 1647658..1647858 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG579_RS08510 1647989..1648672 + 684 WP_006248824.1 helix-turn-helix transcriptional regulator -
BG579_RS08515 1648747..1649127 + 381 WP_006248823.1 hypothetical protein -
BG579_RS08520 1649132..1649617 + 486 WP_015484250.1 hypothetical protein -
BG579_RS08525 1649749..1649931 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG579_RS08530 1649970..1650389 + 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG579_RS08535 1650725..1651369 + 645 WP_006253274.1 tyrosine-type recombinase/integrase -
BG579_RS08545 1651507..1652547 + 1041 WP_045791103.1 IS481 family transposase -
BG579_RS08550 1652614..1654020 - 1407 WP_006253513.1 YdgA family protein -
BG579_RS08555 1654070..1654876 - 807 WP_006248821.1 tryptophan synthase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1651507..1652547 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67226 WP_006250075.1 NZ_CP017528:1649749-1649931 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67226 NZ_CP017528:1649749-1649931 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT67226 WP_006250074.1 NZ_CP017528:1649970-1650389 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT67226 NZ_CP017528:1649970-1650389 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References