Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1283247..1283895 Replicon chromosome
Accession NZ_CP017528
Organism Mannheimia haemolytica strain 1561

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG579_RS06630 Protein ID WP_006251221.1
Coordinates 1283719..1283895 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG579_RS06625 Protein ID WP_006251222.1
Coordinates 1283247..1283663 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG579_RS06590 1279495..1279929 + 435 WP_006251230.1 hypothetical protein -
BG579_RS06595 1279944..1280933 + 990 WP_006251229.1 encapsulin -
BG579_RS13930 1280945..1281250 + 306 WP_015587049.1 hypothetical protein -
BG579_RS06600 1281253..1281630 + 378 WP_006251227.1 hypothetical protein -
BG579_RS06605 1281630..1281974 + 345 WP_006251226.1 hypothetical protein -
BG579_RS06610 1281976..1282344 + 369 WP_006251225.1 hypothetical protein -
BG579_RS06615 1282341..1282721 + 381 WP_006251224.1 hypothetical protein -
BG579_RS06620 1282725..1283207 + 483 WP_006251223.1 phage tail protein -
BG579_RS06625 1283247..1283663 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG579_RS06630 1283719..1283895 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG579_RS06635 1283991..1284662 + 672 WP_006251220.1 hypothetical protein -
BG579_RS06640 1284677..1284910 - 234 WP_020824316.1 hypothetical protein -
BG579_RS06645 1285079..1285537 + 459 WP_006251218.1 hypothetical protein -
BG579_RS06655 1286082..1288577 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1281788..1457033 175245


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67225 WP_006251221.1 NZ_CP017528:c1283895-1283719 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67225 NZ_CP017528:c1283895-1283719 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67225 WP_006251222.1 NZ_CP017528:c1283663-1283247 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67225 NZ_CP017528:c1283663-1283247 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References