Detailed information of TA system TA067219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1856088..1856728
• Replicon: chromosome
• Accession: NZ_CP017527
• Organism: Mannheimia haemolytica strain 1563

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG590_RS09650
• Protein ID: WP_006250075.1
• Coordinates: 1856546..1856728 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG590_RS09645
• Protein ID: WP_006250074.1
• Coordinates: 1856088..1856507 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG590_RS09620	1851601..1852407	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
BG590_RS09625	1852457..1853863	+	1407	WP_006253513.1	YdgA family protein	-
BG590_RS09630	1853930..1854970	-	1041	WP_015587044.1	IS481 family transposase	-
BG590_RS09640	1855108..1855752	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG590_RS09645	1856088..1856507	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG590_RS09650	1856546..1856728	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG590_RS09655	1856860..1857345	-	486	WP_015484250.1	hypothetical protein	-
BG590_RS09660	1857350..1857730	-	381	WP_006248823.1	hypothetical protein	-
BG590_RS09665	1857805..1858488	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG590_RS09670	1858619..1858819	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG590_RS14000	1858840..1859907	+	1068	WP_006253284.1	hypothetical protein	-
BG590_RS14005	1859917..1860075	+	159	WP_006247809.1	hypothetical protein	-
BG590_RS09685	1860084..1860383	-	300	WP_006247810.1	XRE family transcriptional regulator	-
BG590_RS09690	1860355..1860744	-	390	WP_006247811.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG590_RS09695	1860888..1861181	+	294	WP_006250050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1798090..1866571	68481
	-	flank	IS/Tn	-	-	1853930..1854970	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67219 WP_006250075.1 NZ_CP017527:c1856728-1856546 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67219 NZ_CP017527:c1856728-1856546 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67219 WP_006250074.1 NZ_CP017527:c1856507-1856088 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67219 NZ_CP017527:c1856507-1856088 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9