Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1588097..1588745 Replicon chromosome
Accession NZ_CP017527
Organism Mannheimia haemolytica strain 1563

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG590_RS08050 Protein ID WP_006251221.1
Coordinates 1588097..1588273 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG590_RS08055 Protein ID WP_006251222.1
Coordinates 1588329..1588745 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG590_RS08025 1583415..1585910 - 2496 WP_006251216.1 phage tail length tape measure family protein -
BG590_RS08035 1586455..1586913 - 459 WP_006251218.1 hypothetical protein -
BG590_RS08040 1587082..1587315 + 234 WP_020824316.1 hypothetical protein -
BG590_RS08045 1587330..1588001 - 672 WP_006251220.1 hypothetical protein -
BG590_RS08050 1588097..1588273 + 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG590_RS08055 1588329..1588745 + 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG590_RS08060 1588785..1589267 - 483 WP_006251223.1 phage tail protein -
BG590_RS08065 1589271..1589651 - 381 WP_006251224.1 hypothetical protein -
BG590_RS08070 1589648..1590016 - 369 WP_006251225.1 hypothetical protein -
BG590_RS08075 1590018..1590362 - 345 WP_006251226.1 hypothetical protein -
BG590_RS08080 1590362..1590739 - 378 WP_006251227.1 hypothetical protein -
BG590_RS08085 1590742..1591038 - 297 WP_020828758.1 hypothetical protein -
BG590_RS08090 1591050..1592039 - 990 WP_006251229.1 encapsulin -
BG590_RS08095 1592054..1592488 - 435 WP_006251230.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1571008..1664720 93712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67217 WP_006251221.1 NZ_CP017527:1588097-1588273 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67217 NZ_CP017527:1588097-1588273 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67217 WP_006251222.1 NZ_CP017527:1588329-1588745 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67217 NZ_CP017527:1588329-1588745 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References