Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 2023631..2024271 Replicon chromosome
Accession NZ_CP017525
Organism Mannheimia haemolytica strain 1571

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG559_RS10210 Protein ID WP_006250075.1
Coordinates 2023631..2023813 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG559_RS10215 Protein ID WP_006250984.1
Coordinates 2023852..2024271 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG559_RS10175 2019202..2020248 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG559_RS10180 2020245..2021084 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG559_RS10185 2021260..2021520 - 261 WP_006252483.1 hypothetical protein -
BG559_RS10190 2021541..2021741 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG559_RS10195 2021872..2022555 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG559_RS10200 2022630..2023010 + 381 WP_006248823.1 hypothetical protein -
BG559_RS10205 2023015..2023500 + 486 WP_006252741.1 hypothetical protein -
BG559_RS10210 2023631..2023813 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG559_RS10215 2023852..2024271 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG559_RS10220 2024338..2024643 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG559_RS10225 2024652..2024927 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG559_RS10230 2025217..2025447 + 231 WP_006250987.1 hypothetical protein -
BG559_RS10235 2025926..2026249 - 324 WP_006250988.1 hypothetical protein -
BG559_RS10240 2026442..2026627 + 186 WP_020824222.1 hypothetical protein -
BG559_RS13490 2026611..2026775 + 165 WP_020824223.1 hypothetical protein -
BG559_RS10245 2026781..2027239 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG559_RS10250 2027275..2027634 + 360 WP_006250991.1 hypothetical protein -
BG559_RS10255 2027706..2028509 + 804 WP_020824224.1 DUF2303 family protein -
BG559_RS10260 2028603..2029037 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1981079..2034122 53043


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67204 WP_006250075.1 NZ_CP017525:2023631-2023813 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67204 NZ_CP017525:2023631-2023813 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67204 WP_006250984.1 NZ_CP017525:2023852-2024271 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67204 NZ_CP017525:2023852-2024271 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References