Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2481219..2481808 Replicon chromosome
Accession NZ_CP017524
Organism Mannheimia haemolytica strain 1576

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag BG595_RS12795 Protein ID WP_006248516.1
Coordinates 2481476..2481808 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag BG595_RS12790 Protein ID WP_006248517.1
Coordinates 2481219..2481479 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG595_RS12765 2476398..2477543 - 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -
BG595_RS12770 2478155..2478799 + 645 WP_006248521.1 RNA pyrophosphohydrolase -
BG595_RS12775 2478801..2479589 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
BG595_RS12780 2479597..2480394 + 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
BG595_RS12785 2480481..2481149 + 669 WP_006248518.1 phosphoglycolate phosphatase -
BG595_RS12790 2481219..2481479 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG595_RS12795 2481476..2481808 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BG595_RS12800 2481935..2482966 + 1032 WP_006248514.1 tryptophan--tRNA ligase -
BG595_RS12805 2483030..2483785 - 756 WP_006248513.1 M48 family metallopeptidase -
BG595_RS12810 2483841..2484368 - 528 WP_006248512.1 inorganic diphosphatase -
BG595_RS12830 2484948..2485982 + 1035 WP_006248509.1 endolytic transglycosylase MltG -
BG595_RS12835 2485993..2486613 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T67199 WP_006248516.1 NZ_CP017524:2481476-2481808 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T67199 NZ_CP017524:2481476-2481808 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT67199 WP_006248517.1 NZ_CP017524:2481219-2481479 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT67199 NZ_CP017524:2481219-2481479 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References