Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1608606..1609246 Replicon chromosome
Accession NZ_CP017523
Organism Mannheimia haemolytica strain 1582

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG606_RS08215 Protein ID WP_006250075.1
Coordinates 1609064..1609246 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag BG606_RS08210 Protein ID WP_006250074.1
Coordinates 1608606..1609025 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG606_RS08155 1603714..1603959 - 246 WP_006250098.1 hypothetical protein -
BG606_RS13665 1604102..1604272 - 171 WP_006250096.1 hypothetical protein -
BG606_RS08165 1604298..1604483 - 186 WP_006251707.1 hypothetical protein -
BG606_RS08175 1604803..1605276 - 474 WP_006250094.1 hypothetical protein -
BG606_RS08180 1605266..1605835 - 570 WP_006250093.1 recombination protein NinG -
BG606_RS08185 1605963..1606157 - 195 WP_006250091.1 hypothetical protein -
BG606_RS08195 1606448..1607488 - 1041 WP_015586954.1 IS481 family transposase -
BG606_RS08205 1607626..1608270 - 645 WP_006253274.1 tyrosine-type recombinase/integrase -
BG606_RS08210 1608606..1609025 - 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG606_RS08215 1609064..1609246 - 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG606_RS08220 1609378..1609863 - 486 WP_015484280.1 hypothetical protein -
BG606_RS08225 1609868..1610248 - 381 WP_006248823.1 hypothetical protein -
BG606_RS08230 1610323..1611006 - 684 WP_006248824.1 helix-turn-helix transcriptional regulator -
BG606_RS08235 1611137..1611337 + 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG606_RS13670 1611358..1612425 + 1068 WP_006253284.1 hypothetical protein -
BG606_RS13675 1612435..1612593 + 159 WP_006247809.1 hypothetical protein -
BG606_RS08250 1612602..1612901 - 300 WP_006247810.1 XRE family transcriptional regulator -
BG606_RS08255 1612873..1613262 - 390 WP_015484247.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG606_RS08260 1613406..1613699 + 294 WP_006247812.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1572336..1645147 72811
- flank IS/Tn - - 1606448..1607488 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67190 WP_006250075.1 NZ_CP017523:c1609246-1609064 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67190 NZ_CP017523:c1609246-1609064 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT67190 WP_006250074.1 NZ_CP017523:c1609025-1608606 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT67190 NZ_CP017523:c1609025-1608606 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References