Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1273238..1273886 Replicon chromosome
Accession NZ_CP017523
Organism Mannheimia haemolytica strain 1582

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG606_RS06545 Protein ID WP_006251221.1
Coordinates 1273710..1273886 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG606_RS06540 Protein ID WP_006251222.1
Coordinates 1273238..1273654 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG606_RS06500 1269558..1269992 + 435 WP_006251230.1 hypothetical protein -
BG606_RS06505 1270007..1270996 + 990 WP_006251229.1 encapsulin -
BG606_RS06510 1271008..1271241 + 234 WP_075271287.1 hypothetical protein -
BG606_RS06515 1271244..1271621 + 378 WP_006251227.1 hypothetical protein -
BG606_RS06520 1271621..1271965 + 345 WP_006251226.1 hypothetical protein -
BG606_RS06525 1271967..1272335 + 369 WP_006251225.1 hypothetical protein -
BG606_RS06530 1272332..1272712 + 381 WP_006251224.1 hypothetical protein -
BG606_RS06535 1272716..1273198 + 483 WP_006251223.1 phage tail protein -
BG606_RS06540 1273238..1273654 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG606_RS06545 1273710..1273886 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG606_RS06550 1273982..1274653 + 672 WP_006251220.1 hypothetical protein -
BG606_RS06555 1274668..1274901 - 234 WP_020824316.1 hypothetical protein -
BG606_RS06560 1275070..1275528 + 459 WP_006251218.1 hypothetical protein -
BG606_RS06570 1276073..1278568 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1235575..1297674 62099


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67189 WP_006251221.1 NZ_CP017523:c1273886-1273710 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67189 NZ_CP017523:c1273886-1273710 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67189 WP_006251222.1 NZ_CP017523:c1273654-1273238 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67189 NZ_CP017523:c1273654-1273238 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References