Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 795553..796075 Replicon chromosome
Accession NZ_CP017523
Organism Mannheimia haemolytica strain 1582

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag BG606_RS03975 Protein ID WP_006249269.1
Coordinates 795553..795849 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag BG606_RS03980 Protein ID WP_006249270.1
Coordinates 795833..796075 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG606_RS03960 790608..791933 + 1326 WP_006249264.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -
BG606_RS03965 792054..792353 + 300 WP_006249265.1 hypothetical protein -
BG606_RS03970 792514..795426 + 2913 WP_147009619.1 RNA polymerase-associated protein RapA -
BG606_RS03975 795553..795849 + 297 WP_006249269.1 BrnT family toxin Toxin
BG606_RS03980 795833..796075 + 243 WP_006249270.1 CopG family transcriptional regulator Antitoxin
BG606_RS03985 796087..796755 + 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
BG606_RS03990 796733..797308 - 576 WP_147009620.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
BG606_RS03995 797368..798210 + 843 WP_006249273.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
BG606_RS04000 798333..798974 + 642 WP_006249274.1 orotate phosphoribosyltransferase -
BG606_RS04005 799148..800237 + 1090 Protein_766 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - htrB 661721..816804 155083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T67188 WP_006249269.1 NZ_CP017523:795553-795849 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T67188 NZ_CP017523:795553-795849 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT67188 WP_006249270.1 NZ_CP017523:795833-796075 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT67188 NZ_CP017523:795833-796075 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References