Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 2010503..2011143 Replicon chromosome
Accession NZ_CP017522
Organism Mannheimia haemolytica strain 1583

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG545_RS10090 Protein ID WP_006250075.1
Coordinates 2010503..2010685 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG545_RS10095 Protein ID WP_006250984.1
Coordinates 2010724..2011143 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG545_RS10055 2006074..2007120 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG545_RS10060 2007117..2007956 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG545_RS10065 2008132..2008392 - 261 WP_006252483.1 hypothetical protein -
BG545_RS10070 2008413..2008613 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG545_RS10075 2008744..2009427 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG545_RS10080 2009502..2009882 + 381 WP_006248823.1 hypothetical protein -
BG545_RS10085 2009887..2010372 + 486 WP_006252741.1 hypothetical protein -
BG545_RS10090 2010503..2010685 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG545_RS10095 2010724..2011143 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG545_RS10100 2011210..2011515 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG545_RS10105 2011524..2011799 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG545_RS10110 2012089..2012319 + 231 WP_006250987.1 hypothetical protein -
BG545_RS10115 2012798..2013121 - 324 WP_006250988.1 hypothetical protein -
BG545_RS10120 2013314..2013499 + 186 WP_020824222.1 hypothetical protein -
BG545_RS13365 2013483..2013647 + 165 WP_020824223.1 hypothetical protein -
BG545_RS10125 2013653..2014111 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG545_RS10130 2014147..2014506 + 360 WP_006250991.1 hypothetical protein -
BG545_RS10135 2014578..2015381 + 804 WP_020824224.1 DUF2303 family protein -
BG545_RS10140 2015475..2015909 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1943311..2021491 78180


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67183 WP_006250075.1 NZ_CP017522:2010503-2010685 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67183 NZ_CP017522:2010503-2010685 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67183 WP_006250984.1 NZ_CP017522:2010724-2011143 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67183 NZ_CP017522:2010724-2011143 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References