Detailed information of TA system TA067182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1600849..1601497
• Replicon: chromosome
• Accession: NZ_CP017522
• Organism: Mannheimia haemolytica strain 1583

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG545_RS08095
• Protein ID: WP_006251221.1
• Coordinates: 1601321..1601497 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG545_RS08090
• Protein ID: WP_006251222.1
• Coordinates: 1600849..1601265 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG545_RS08050	1597145..1597579	+	435	WP_006251230.1	hypothetical protein	-
BG545_RS08055	1597594..1598580	+	987	WP_062627922.1	encapsulin	-
BG545_RS08060	1598592..1598852	+	261	WP_020824317.1	hypothetical protein	-
BG545_RS08065	1598855..1599232	+	378	WP_006251227.1	hypothetical protein	-
BG545_RS08070	1599232..1599576	+	345	WP_006251226.1	hypothetical protein	-
BG545_RS08075	1599578..1599946	+	369	WP_006251225.1	hypothetical protein	-
BG545_RS08080	1599943..1600323	+	381	WP_006251224.1	hypothetical protein	-
BG545_RS08085	1600327..1600809	+	483	WP_006251223.1	phage tail protein	-
BG545_RS08090	1600849..1601265	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG545_RS08095	1601321..1601497	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG545_RS08100	1601594..1602265	+	672	WP_006251220.1	hypothetical protein	-
BG545_RS08105	1602280..1602513	-	234	WP_020824316.1	hypothetical protein	-
BG545_RS08110	1602682..1603140	+	459	WP_006251218.1	hypothetical protein	-
BG545_RS08115	1603208..1603603	+	396	WP_006251217.1	hypothetical protein	-
BG545_RS08120	1603690..1606185	+	2496	WP_061888644.1	phage tail length tape measure family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1566691..1615171	48480

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67182 WP_006251221.1 NZ_CP017522:c1601497-1601321 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67182 NZ_CP017522:c1601497-1601321 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67182 WP_006251222.1 NZ_CP017522:c1601265-1600849 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67182 NZ_CP017522:c1601265-1600849 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6