Detailed information of TA system TA067175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1683263..1683903
• Replicon: chromosome
• Accession: NZ_CP017521
• Organism: Mannheimia haemolytica strain 1584

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG594_RS08735
• Protein ID: WP_006250075.1
• Coordinates: 1683263..1683445 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG594_RS08740
• Protein ID: WP_006250074.1
• Coordinates: 1683484..1683903 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG594_RS08690	1678810..1679103	-	294	WP_006247812.1	hypothetical protein	-
BG594_RS08695	1679247..1679636	+	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG594_RS08700	1679608..1679907	+	300	WP_006247810.1	XRE family transcriptional regulator	-
BG594_RS14275	1679916..1680074	-	159	WP_006247809.1	hypothetical protein	-
BG594_RS14280	1680084..1681151	-	1068	WP_006253284.1	hypothetical protein	-
BG594_RS08715	1681172..1681372	-	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG594_RS08720	1681503..1682186	+	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG594_RS08725	1682261..1682641	+	381	WP_006248823.1	hypothetical protein	-
BG594_RS08730	1682646..1683131	+	486	WP_015484250.1	hypothetical protein	-
BG594_RS08735	1683263..1683445	+	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG594_RS08740	1683484..1683903	+	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG594_RS08745	1684239..1684883	+	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG594_RS08755	1685021..1686061	+	1041	WP_096864247.1	IS481 family transposase	-
BG594_RS08760	1686128..1687534	-	1407	WP_006253513.1	YdgA family protein	-
BG594_RS08765	1687584..1688390	-	807	WP_006248821.1	tryptophan synthase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1601560..1741901	140341
	-	flank	IS/Tn	-	-	1685021..1686061	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67175 WP_006250075.1 NZ_CP017521:1683263-1683445 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67175 NZ_CP017521:1683263-1683445 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67175 WP_006250074.1 NZ_CP017521:1683484-1683903 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67175 NZ_CP017521:1683484-1683903 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9