Detailed information of TA system TA067159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1128837..1129477
• Replicon: chromosome
• Accession: NZ_CP017519
• Organism: Mannheimia haemolytica strain 22549

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG610_RS05770
• Protein ID: WP_006250075.1
• Coordinates: 1129295..1129477 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG610_RS05765
• Protein ID: WP_006250074.1
• Coordinates: 1128837..1129256 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG610_RS05745	1124972..1125778	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
BG610_RS05750	1125828..1127234	+	1407	WP_006253513.1	YdgA family protein	-
BG610_RS05755	1127301..1128341	-	1041	WP_015587043.1	IS481 family transposase	-
BG610_RS05765	1128837..1129256	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG610_RS05770	1129295..1129477	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG610_RS05775	1129609..1130094	-	486	WP_015484250.1	hypothetical protein	-
BG610_RS05780	1130099..1130479	-	381	WP_006248823.1	hypothetical protein	-
BG610_RS05785	1130554..1131237	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG610_RS05790	1131368..1131568	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG610_RS13910	1131589..1132656	+	1068	WP_006253284.1	hypothetical protein	-
BG610_RS13915	1132666..1132824	+	159	WP_006247809.1	hypothetical protein	-
BG610_RS05805	1132884..1133924	-	1041	WP_015587043.1	IS481 family transposase	-
BG610_RS05815	1134041..1134340	-	300	WP_006247810.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1071876..1138281	66405
	-	inside	IScluster/Tn	-	-	1127301..1133924	6623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67159 WP_006250075.1 NZ_CP017519:c1129477-1129295 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67159 NZ_CP017519:c1129477-1129295 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67159 WP_006250074.1 NZ_CP017519:c1129256-1128837 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67159 NZ_CP017519:c1129256-1128837 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9