Detailed information of TA system TA067154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2335952..2336600
• Replicon: chromosome
• Accession: NZ_CP017518
• Organism: Mannheimia haemolytica strain 22604

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG563_RS11765
• Protein ID: WP_006251221.1
• Coordinates: 2335952..2336128 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG563_RS11770
• Protein ID: WP_006251222.1
• Coordinates: 2336184..2336600 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG563_RS11740	2331264..2333759	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG563_RS11745	2333846..2334241	-	396	WP_006251217.1	hypothetical protein	-
BG563_RS11750	2334309..2334767	-	459	WP_006251218.1	hypothetical protein	-
BG563_RS11755	2334936..2335169	+	234	WP_020824316.1	hypothetical protein	-
BG563_RS11760	2335184..2335855	-	672	WP_006251220.1	hypothetical protein	-
BG563_RS11765	2335952..2336128	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG563_RS11770	2336184..2336600	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG563_RS11775	2336640..2337122	-	483	WP_006251223.1	phage tail protein	-
BG563_RS11780	2337126..2337506	-	381	WP_006251224.1	hypothetical protein	-
BG563_RS11785	2337503..2337871	-	369	WP_006251225.1	hypothetical protein	-
BG563_RS11790	2337873..2338217	-	345	WP_006251226.1	hypothetical protein	-
BG563_RS11795	2338217..2338594	-	378	WP_006251227.1	hypothetical protein	-
BG563_RS11800	2338597..2338821	-	225	WP_021265457.1	hypothetical protein	-
BG563_RS11805	2338833..2339819	-	987	WP_062627922.1	encapsulin	-
BG563_RS11810	2339834..2340268	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2322278..2370722	48444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67154 WP_006251221.1 NZ_CP017518:2335952-2336128 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67154 NZ_CP017518:2335952-2336128 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67154 WP_006251222.1 NZ_CP017518:2336184-2336600 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67154 NZ_CP017518:2336184-2336600 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6