67153

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 2182043..2182565 Replicon chromosome
Accession NZ_CP017518
Organism Mannheimia haemolytica strain 22604

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZX63
Locus tag BG563_RS10920 Protein ID WP_006250322.1
Coordinates 2182043..2182312 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A248ZY54
Locus tag BG563_RS10925 Protein ID WP_006250323.1
Coordinates 2182299..2182565 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG563_RS10900 2177363..2177797 - 435 WP_006250318.1 D-tyrosyl-tRNA(Tyr) deacylase -
BG563_RS10905 2177772..2178617 - 846 WP_006251985.1 virulence factor BrkB family protein -
BG563_RS10910 2178619..2179857 - 1239 WP_006251984.1 glutamate-5-semialdehyde dehydrogenase -
BG563_RS10915 2180141..2182024 + 1884 WP_006251983.1 DNA mismatch repair endonuclease MutL -
BG563_RS10920 2182043..2182312 - 270 WP_006250322.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG563_RS10925 2182299..2182565 - 267 WP_006250323.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG563_RS10930 2183011..2183964 + 954 WP_006251982.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
BG563_RS10935 2184033..2184305 + 273 WP_006250326.1 RNA chaperone Hfq -
BG563_RS10940 2184325..2185710 + 1386 WP_020824252.1 GTPase HflX -
BG563_RS10950 2186047..2187087 + 1041 WP_020824044.1 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2186047..2187087 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10447.99 Da        Isoelectric Point: 6.4732

>T67153 WP_006250322.1 NZ_CP017518:c2182312-2182043 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Download         Length: 270 bp

>T67153 NZ_CP017518:c2182312-2182043 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GTTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9738.10 Da        Isoelectric Point: 4.7954

>AT67153 WP_006250323.1 NZ_CP017518:c2182565-2182299 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Download         Length: 267 bp

>AT67153 NZ_CP017518:c2182565-2182299 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZX63

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZY54

References