Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1995279..1995919 Replicon chromosome
Accession NZ_CP017518
Organism Mannheimia haemolytica strain 22604

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG563_RS09895 Protein ID WP_006250075.1
Coordinates 1995279..1995461 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG563_RS09900 Protein ID WP_006250984.1
Coordinates 1995500..1995919 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG563_RS09860 1990850..1991896 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG563_RS09865 1991893..1992732 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG563_RS09870 1992908..1993168 - 261 WP_006252483.1 hypothetical protein -
BG563_RS09875 1993189..1993389 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG563_RS09880 1993520..1994203 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG563_RS09885 1994278..1994658 + 381 WP_006248823.1 hypothetical protein -
BG563_RS09890 1994663..1995148 + 486 WP_006252741.1 hypothetical protein -
BG563_RS09895 1995279..1995461 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG563_RS09900 1995500..1995919 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG563_RS09905 1995986..1996291 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG563_RS09910 1996300..1996575 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG563_RS09915 1996865..1997095 + 231 WP_006250987.1 hypothetical protein -
BG563_RS09920 1997574..1997897 - 324 WP_006250988.1 hypothetical protein -
BG563_RS09925 1998090..1998275 + 186 WP_020824222.1 hypothetical protein -
BG563_RS13365 1998259..1998423 + 165 WP_020824223.1 hypothetical protein -
BG563_RS09930 1998429..1998887 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG563_RS09935 1998923..1999282 + 360 WP_006250991.1 hypothetical protein -
BG563_RS09940 1999354..2000157 + 804 WP_020824224.1 DUF2303 family protein -
BG563_RS09945 2000209..2000643 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1952720..2005728 53008


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67152 WP_006250075.1 NZ_CP017518:1995279-1995461 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67152 NZ_CP017518:1995279-1995461 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67152 WP_006250984.1 NZ_CP017518:1995500-1995919 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67152 NZ_CP017518:1995500-1995919 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References