Detailed information of TA system TA067147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2335955..2336603
• Replicon: chromosome
• Accession: NZ_CP017517
• Organism: Mannheimia haemolytica strain 22650

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG564_RS11765
• Protein ID: WP_006251221.1
• Coordinates: 2335955..2336131 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG564_RS11770
• Protein ID: WP_006251222.1
• Coordinates: 2336187..2336603 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG564_RS11740	2331267..2333762	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG564_RS11745	2333849..2334244	-	396	WP_006251217.1	hypothetical protein	-
BG564_RS11750	2334312..2334770	-	459	WP_006251218.1	hypothetical protein	-
BG564_RS11755	2334939..2335172	+	234	WP_020824316.1	hypothetical protein	-
BG564_RS11760	2335187..2335858	-	672	WP_006251220.1	hypothetical protein	-
BG564_RS11765	2335955..2336131	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG564_RS11770	2336187..2336603	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG564_RS11775	2336643..2337125	-	483	WP_006251223.1	phage tail protein	-
BG564_RS11780	2337129..2337509	-	381	WP_006251224.1	hypothetical protein	-
BG564_RS11785	2337506..2337874	-	369	WP_006251225.1	hypothetical protein	-
BG564_RS11790	2337876..2338220	-	345	WP_006251226.1	hypothetical protein	-
BG564_RS11795	2338220..2338597	-	378	WP_006251227.1	hypothetical protein	-
BG564_RS11800	2338600..2338824	-	225	WP_021265457.1	hypothetical protein	-
BG564_RS11805	2338836..2339822	-	987	WP_062627922.1	encapsulin	-
BG564_RS11810	2339837..2340271	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2322281..2370725	48444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67147 WP_006251221.1 NZ_CP017517:2335955-2336131 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67147 NZ_CP017517:2335955-2336131 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67147 WP_006251222.1 NZ_CP017517:2336187-2336603 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67147 NZ_CP017517:2336187-2336603 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6