Detailed information of TA system TA067140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2306325..2306973
• Replicon: chromosome
• Accession: NZ_CP017516
• Organism: Mannheimia haemolytica strain 23217

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG560_RS11635
• Protein ID: WP_006251221.1
• Coordinates: 2306325..2306501 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG560_RS11640
• Protein ID: WP_006251222.1
• Coordinates: 2306557..2306973 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG560_RS11610	2301638..2304133	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG560_RS11615	2304220..2304615	-	396	WP_006251217.1	hypothetical protein	-
BG560_RS11620	2304683..2305141	-	459	WP_006251218.1	hypothetical protein	-
BG560_RS11625	2305274..2305543	+	270	WP_006251219.1	hypothetical protein	-
BG560_RS11630	2305558..2306229	-	672	WP_006251220.1	hypothetical protein	-
BG560_RS11635	2306325..2306501	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG560_RS11640	2306557..2306973	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG560_RS11645	2307013..2307495	-	483	WP_006251223.1	phage tail protein	-
BG560_RS11650	2307499..2307879	-	381	WP_006251224.1	hypothetical protein	-
BG560_RS11655	2307876..2308244	-	369	WP_061888643.1	hypothetical protein	-
BG560_RS11660	2308246..2308590	-	345	WP_006251226.1	hypothetical protein	-
BG560_RS11665	2308590..2308967	-	378	WP_006251227.1	hypothetical protein	-
BG560_RS11670	2308970..2309167	-	198	WP_032848903.1	hypothetical protein	-
BG560_RS11675	2309179..2310168	-	990	WP_006251229.1	encapsulin	-
BG560_RS11680	2310183..2310617	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2292652..2328773	36121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67140 WP_006251221.1 NZ_CP017516:2306325-2306501 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67140 NZ_CP017516:2306325-2306501 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67140 WP_006251222.1 NZ_CP017516:2306557-2306973 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67140 NZ_CP017516:2306557-2306973 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6