Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 2150140..2150662 Replicon chromosome
Accession NZ_CP017515
Organism Mannheimia haemolytica strain 23278

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZX63
Locus tag BG546_RS10780 Protein ID WP_006250322.1
Coordinates 2150140..2150409 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A248ZY54
Locus tag BG546_RS10785 Protein ID WP_006250323.1
Coordinates 2150396..2150662 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG546_RS10760 2145460..2145894 - 435 WP_006250318.1 D-tyrosyl-tRNA(Tyr) deacylase -
BG546_RS10765 2145869..2146714 - 846 WP_006251985.1 virulence factor BrkB family protein -
BG546_RS10770 2146716..2147954 - 1239 WP_006251984.1 glutamate-5-semialdehyde dehydrogenase -
BG546_RS10775 2148238..2150121 + 1884 WP_006251983.1 DNA mismatch repair endonuclease MutL -
BG546_RS10780 2150140..2150409 - 270 WP_006250322.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG546_RS10785 2150396..2150662 - 267 WP_006250323.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG546_RS10790 2151108..2152061 + 954 WP_006251982.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
BG546_RS10795 2152130..2152402 + 273 WP_006250326.1 RNA chaperone Hfq -
BG546_RS10800 2152422..2153807 + 1386 WP_020824252.1 GTPase HflX -
BG546_RS10810 2154144..2155184 + 1041 WP_020824044.1 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2154144..2155184 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10447.99 Da        Isoelectric Point: 6.4732

>T67133 WP_006250322.1 NZ_CP017515:c2150409-2150140 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Download         Length: 270 bp

>T67133 NZ_CP017515:c2150409-2150140 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GTTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9738.10 Da        Isoelectric Point: 4.7954

>AT67133 WP_006250323.1 NZ_CP017515:c2150662-2150396 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Download         Length: 267 bp

>AT67133 NZ_CP017515:c2150662-2150396 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZX63

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZY54

References