Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1963334..1963974 Replicon chromosome
Accession NZ_CP017515
Organism Mannheimia haemolytica strain 23278

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG546_RS09755 Protein ID WP_006250075.1
Coordinates 1963334..1963516 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG546_RS09760 Protein ID WP_006250984.1
Coordinates 1963555..1963974 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG546_RS09720 1958905..1959951 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG546_RS09725 1959948..1960787 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG546_RS09730 1960963..1961223 - 261 WP_006252483.1 hypothetical protein -
BG546_RS09735 1961244..1961444 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG546_RS09740 1961575..1962258 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG546_RS09745 1962333..1962713 + 381 WP_006248823.1 hypothetical protein -
BG546_RS09750 1962718..1963203 + 486 WP_006252741.1 hypothetical protein -
BG546_RS09755 1963334..1963516 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG546_RS09760 1963555..1963974 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG546_RS09765 1964041..1964346 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG546_RS09770 1964355..1964630 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG546_RS09775 1964920..1965150 + 231 WP_006250987.1 hypothetical protein -
BG546_RS09780 1965629..1965952 - 324 WP_006250988.1 hypothetical protein -
BG546_RS09785 1966145..1966330 + 186 WP_020824222.1 hypothetical protein -
BG546_RS13010 1966314..1966478 + 165 WP_020824223.1 hypothetical protein -
BG546_RS09790 1966484..1966942 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG546_RS09795 1966978..1967337 + 360 WP_006250991.1 hypothetical protein -
BG546_RS09800 1967409..1968212 + 804 WP_020824224.1 DUF2303 family protein -
BG546_RS09805 1968306..1968740 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1902344..1973825 71481


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67132 WP_006250075.1 NZ_CP017515:1963334-1963516 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67132 NZ_CP017515:1963334-1963516 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67132 WP_006250984.1 NZ_CP017515:1963555-1963974 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67132 NZ_CP017515:1963555-1963974 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References