Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 490948..491598 Replicon chromosome
Accession NZ_CP017515
Organism Mannheimia haemolytica strain 23278

Toxin (Protein)


Gene name vapC Uniprot ID A0A378NBZ4
Locus tag BG546_RS02435 Protein ID WP_020831127.1
Coordinates 491209..491598 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D2Q6P1
Locus tag BG546_RS02430 Protein ID WP_006251766.1
Coordinates 490948..491208 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG546_RS02415 486636..490169 + 3534 WP_006251763.1 transcription-repair coupling factor -
BG546_RS02420 490332..490619 + 288 WP_020831126.1 BrnT family toxin -
BG546_RS02425 490620..490832 + 213 WP_006251765.1 BrnA antitoxin family protein -
BG546_RS02430 490948..491208 + 261 WP_006251766.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG546_RS02435 491209..491598 + 390 WP_020831127.1 type II toxin-antitoxin system VapC family toxin Toxin
BG546_RS02440 491618..492856 + 1239 WP_006251767.1 peptidase T -
BG546_RS02480 493846..494631 + 786 WP_006251768.1 hypothetical protein -
BG546_RS02485 494705..495565 + 861 WP_006250124.1 metal ABC transporter permease -
BG546_RS02490 495558..496367 + 810 WP_006250123.1 metal ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14575.87 Da        Isoelectric Point: 7.7391

>T67130 WP_020831127.1 NZ_CP017515:491209-491598 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLGYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T67130 NZ_CP017515:491209-491598 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGGTTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.50 Da        Isoelectric Point: 4.6909

>AT67130 WP_006251766.1 NZ_CP017515:490948-491208 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT67130 NZ_CP017515:490948-491208 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NBZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D2Q6P1

References