Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1981983..1982623 Replicon chromosome
Accession NZ_CP017514
Organism Mannheimia haemolytica strain 23311

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG573_RS09790 Protein ID WP_006250075.1
Coordinates 1981983..1982165 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG573_RS09795 Protein ID WP_006250984.1
Coordinates 1982204..1982623 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG573_RS09755 1977554..1978600 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG573_RS09760 1978597..1979436 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG573_RS09765 1979612..1979872 - 261 WP_006252483.1 hypothetical protein -
BG573_RS09770 1979893..1980093 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG573_RS09775 1980224..1980907 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG573_RS09780 1980982..1981362 + 381 WP_006248823.1 hypothetical protein -
BG573_RS09785 1981367..1981852 + 486 WP_006252741.1 hypothetical protein -
BG573_RS09790 1981983..1982165 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG573_RS09795 1982204..1982623 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG573_RS09800 1982690..1982995 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG573_RS09805 1983004..1983279 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG573_RS09810 1983569..1983799 + 231 WP_006250987.1 hypothetical protein -
BG573_RS09815 1984278..1984601 - 324 WP_006250988.1 hypothetical protein -
BG573_RS09820 1984794..1984979 + 186 WP_020824222.1 hypothetical protein -
BG573_RS12775 1984963..1985127 + 165 WP_020824223.1 hypothetical protein -
BG573_RS09825 1985133..1985591 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG573_RS09830 1985627..1985986 + 360 WP_006250991.1 hypothetical protein -
BG573_RS09835 1986058..1986861 + 804 WP_020824224.1 DUF2303 family protein -
BG573_RS09840 1986955..1987389 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1920993..1992971 71978


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67127 WP_006250075.1 NZ_CP017514:1981983-1982165 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67127 NZ_CP017514:1981983-1982165 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67127 WP_006250984.1 NZ_CP017514:1982204-1982623 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67127 NZ_CP017514:1982204-1982623 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A378NFX7


Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References