Detailed information of TA system TA067121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2292357..2293005
• Replicon: chromosome
• Accession: NZ_CP017513
• Organism: Mannheimia haemolytica strain 23533

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG553_RS11685
• Protein ID: WP_006251221.1
• Coordinates: 2292829..2293005 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG553_RS11680
• Protein ID: WP_006251222.1
• Coordinates: 2292357..2292773 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG553_RS11640	2288680..2289114	+	435	WP_006251230.1	hypothetical protein	-
BG553_RS11645	2289129..2290115	+	987	WP_062627922.1	encapsulin	-
BG553_RS11650	2290127..2290360	+	234	WP_075271287.1	hypothetical protein	-
BG553_RS11655	2290363..2290740	+	378	WP_006251227.1	hypothetical protein	-
BG553_RS11660	2290740..2291084	+	345	WP_006251226.1	hypothetical protein	-
BG553_RS11665	2291086..2291454	+	369	WP_006251225.1	hypothetical protein	-
BG553_RS11670	2291451..2291831	+	381	WP_006251224.1	hypothetical protein	-
BG553_RS11675	2291835..2292317	+	483	WP_006251223.1	phage tail protein	-
BG553_RS11680	2292357..2292773	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG553_RS11685	2292829..2293005	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG553_RS11690	2293102..2293773	+	672	WP_006251220.1	hypothetical protein	-
BG553_RS11695	2293788..2294057	-	270	WP_006251219.1	hypothetical protein	-
BG553_RS11700	2294190..2294648	+	459	WP_006251218.1	hypothetical protein	-
BG553_RS11705	2294716..2295111	+	396	WP_006251217.1	hypothetical protein	-
BG553_RS11710	2295198..2297687	+	2490	WP_147009468.1	phage tail length tape measure family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2255897..2306673	50776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67121 WP_006251221.1 NZ_CP017513:c2293005-2292829 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67121 NZ_CP017513:c2293005-2292829 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67121 WP_006251222.1 NZ_CP017513:c2292773-2292357 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67121 NZ_CP017513:c2292773-2292357 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6