Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1958687..1959327 Replicon chromosome
Accession NZ_CP017513
Organism Mannheimia haemolytica strain 23533

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG553_RS09685 Protein ID WP_006250075.1
Coordinates 1958687..1958869 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG553_RS09690 Protein ID WP_006250984.1
Coordinates 1958908..1959327 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG553_RS09650 1954258..1955304 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG553_RS09655 1955301..1956140 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG553_RS09660 1956316..1956576 - 261 WP_006252483.1 hypothetical protein -
BG553_RS09665 1956597..1956797 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG553_RS09670 1956928..1957611 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG553_RS09675 1957686..1958066 + 381 WP_006248823.1 hypothetical protein -
BG553_RS09680 1958071..1958556 + 486 WP_186805239.1 hypothetical protein -
BG553_RS09685 1958687..1958869 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG553_RS09690 1958908..1959327 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG553_RS09695 1959394..1959699 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG553_RS09700 1959708..1959983 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG553_RS09705 1960273..1960503 + 231 WP_006250987.1 hypothetical protein -
BG553_RS09710 1960982..1961305 - 324 WP_006250988.1 hypothetical protein -
BG553_RS09715 1961498..1961683 + 186 WP_020824222.1 hypothetical protein -
BG553_RS13365 1961667..1961831 + 165 WP_020824223.1 hypothetical protein -
BG553_RS09720 1961837..1962295 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG553_RS09725 1962331..1962690 + 360 WP_006250991.1 hypothetical protein -
BG553_RS09730 1962762..1963565 + 804 WP_020824224.1 DUF2303 family protein -
BG553_RS09735 1963659..1964093 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1916128..1969178 53050


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67119 WP_006250075.1 NZ_CP017513:1958687-1958869 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67119 NZ_CP017513:1958687-1958869 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67119 WP_006250984.1 NZ_CP017513:1958908-1959327 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67119 NZ_CP017513:1958908-1959327 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References