Detailed information of TA system TA067107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2292289..2292937
• Replicon: chromosome
• Accession: NZ_CP017511
• Organism: Mannheimia haemolytica strain 23570

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG555_RS11685
• Protein ID: WP_006251221.1
• Coordinates: 2292761..2292937 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG555_RS11680
• Protein ID: WP_006251222.1
• Coordinates: 2292289..2292705 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG555_RS11640	2288612..2289046	+	435	WP_006251230.1	hypothetical protein	-
BG555_RS11645	2289061..2290047	+	987	WP_062627922.1	encapsulin	-
BG555_RS11650	2290059..2290292	+	234	WP_075271287.1	hypothetical protein	-
BG555_RS11655	2290295..2290672	+	378	WP_006251227.1	hypothetical protein	-
BG555_RS11660	2290672..2291016	+	345	WP_006251226.1	hypothetical protein	-
BG555_RS11665	2291018..2291386	+	369	WP_006251225.1	hypothetical protein	-
BG555_RS11670	2291383..2291763	+	381	WP_006251224.1	hypothetical protein	-
BG555_RS11675	2291767..2292249	+	483	WP_006251223.1	phage tail protein	-
BG555_RS11680	2292289..2292705	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG555_RS11685	2292761..2292937	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG555_RS11690	2293034..2293705	+	672	WP_006251220.1	hypothetical protein	-
BG555_RS11695	2293720..2293953	-	234	WP_020824316.1	hypothetical protein	-
BG555_RS11700	2294122..2294580	+	459	WP_006251218.1	hypothetical protein	-
BG555_RS11705	2294648..2295043	+	396	WP_006251217.1	hypothetical protein	-
BG555_RS11710	2295130..2297619	+	2490	WP_147009468.1	phage tail length tape measure family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2250635..2306605	55970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67107 WP_006251221.1 NZ_CP017511:c2292937-2292761 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67107 NZ_CP017511:c2292937-2292761 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67107 WP_006251222.1 NZ_CP017511:c2292705-2292289 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67107 NZ_CP017511:c2292705-2292289 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6