Detailed information of TA system TA067100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2292323..2292971
• Replicon: chromosome
• Accession: NZ_CP017510
• Organism: Mannheimia haemolytica strain 23579

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG556_RS11685
• Protein ID: WP_006251221.1
• Coordinates: 2292795..2292971 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG556_RS11680
• Protein ID: WP_006251222.1
• Coordinates: 2292323..2292739 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG556_RS11640	2288646..2289080	+	435	WP_006251230.1	hypothetical protein	-
BG556_RS11645	2289095..2290081	+	987	WP_062627922.1	encapsulin	-
BG556_RS11650	2290093..2290326	+	234	WP_075271287.1	hypothetical protein	-
BG556_RS11655	2290329..2290706	+	378	WP_006251227.1	hypothetical protein	-
BG556_RS11660	2290706..2291050	+	345	WP_006251226.1	hypothetical protein	-
BG556_RS11665	2291052..2291420	+	369	WP_006251225.1	hypothetical protein	-
BG556_RS11670	2291417..2291797	+	381	WP_006251224.1	hypothetical protein	-
BG556_RS11675	2291801..2292283	+	483	WP_006251223.1	phage tail protein	-
BG556_RS11680	2292323..2292739	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG556_RS11685	2292795..2292971	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG556_RS11690	2293068..2293739	+	672	WP_006251220.1	hypothetical protein	-
BG556_RS11695	2293754..2293987	-	234	WP_020824316.1	hypothetical protein	-
BG556_RS11700	2294156..2294614	+	459	WP_006251218.1	hypothetical protein	-
BG556_RS11705	2294682..2295077	+	396	WP_006251217.1	hypothetical protein	-
BG556_RS11710	2295164..2297653	+	2490	WP_147009468.1	phage tail length tape measure family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2255863..2306639	50776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67100 WP_006251221.1 NZ_CP017510:c2292971-2292795 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67100 NZ_CP017510:c2292971-2292795 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67100 WP_006251222.1 NZ_CP017510:c2292739-2292323 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67100 NZ_CP017510:c2292739-2292323 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6