Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1958653..1959293 Replicon chromosome
Accession NZ_CP017510
Organism Mannheimia haemolytica strain 23579

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG556_RS09685 Protein ID WP_006250075.1
Coordinates 1958653..1958835 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG556_RS09690 Protein ID WP_006250984.1
Coordinates 1958874..1959293 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG556_RS09650 1954224..1955270 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG556_RS09655 1955267..1956106 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG556_RS09660 1956282..1956542 - 261 WP_006252483.1 hypothetical protein -
BG556_RS09665 1956563..1956763 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG556_RS09670 1956894..1957577 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG556_RS09675 1957652..1958032 + 381 WP_006248823.1 hypothetical protein -
BG556_RS09680 1958037..1958522 + 486 WP_186805239.1 hypothetical protein -
BG556_RS09685 1958653..1958835 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG556_RS09690 1958874..1959293 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG556_RS09695 1959360..1959665 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG556_RS09700 1959674..1959949 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG556_RS09705 1960239..1960469 + 231 WP_006250987.1 hypothetical protein -
BG556_RS09710 1960948..1961271 - 324 WP_006250988.1 hypothetical protein -
BG556_RS09715 1961464..1961649 + 186 WP_020824222.1 hypothetical protein -
BG556_RS13375 1961633..1961797 + 165 WP_020824223.1 hypothetical protein -
BG556_RS09720 1961803..1962261 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG556_RS09725 1962297..1962656 + 360 WP_006250991.1 hypothetical protein -
BG556_RS09730 1962728..1963531 + 804 WP_020824224.1 DUF2303 family protein -
BG556_RS09735 1963625..1964059 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1916094..1969144 53050


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67098 WP_006250075.1 NZ_CP017510:1958653-1958835 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67098 NZ_CP017510:1958653-1958835 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67098 WP_006250984.1 NZ_CP017510:1958874-1959293 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67098 NZ_CP017510:1958874-1959293 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References