Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2135500..2136150 Replicon chromosome
Accession NZ_CP017509
Organism Mannheimia haemolytica strain 28226

Toxin (Protein)


Gene name vapC Uniprot ID A0A378NBZ4
Locus tag BG574_RS10880 Protein ID WP_020831127.1
Coordinates 2135500..2135889 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D2Q6P1
Locus tag BG574_RS10885 Protein ID WP_006251766.1
Coordinates 2135890..2136150 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG574_RS10840 2131061..2131870 - 810 WP_006250123.1 metal ABC transporter permease -
BG574_RS10845 2131863..2132723 - 861 WP_006250124.1 metal ABC transporter permease -
BG574_RS10850 2132797..2133582 - 786 WP_006251768.1 hypothetical protein -
BG574_RS10875 2134242..2135480 - 1239 WP_006251767.1 peptidase T -
BG574_RS10880 2135500..2135889 - 390 WP_020831127.1 type II toxin-antitoxin system VapC family toxin Toxin
BG574_RS10885 2135890..2136150 - 261 WP_006251766.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG574_RS10890 2136266..2136478 - 213 WP_006251765.1 BrnA antitoxin family protein -
BG574_RS10895 2136479..2136766 - 288 WP_020831126.1 BrnT family toxin -
BG574_RS10900 2136929..2140462 - 3534 WP_006251763.1 transcription-repair coupling factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14575.87 Da        Isoelectric Point: 7.7391

>T67093 WP_020831127.1 NZ_CP017509:c2135889-2135500 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLGYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T67093 NZ_CP017509:c2135889-2135500 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGGTTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.50 Da        Isoelectric Point: 4.6909

>AT67093 WP_006251766.1 NZ_CP017509:c2136150-2135890 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT67093 NZ_CP017509:c2136150-2135890 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NBZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D2Q6P1

References