Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 697989..698629 Replicon chromosome
Accession NZ_CP017509
Organism Mannheimia haemolytica strain 28226

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG574_RS03825 Protein ID WP_006250075.1
Coordinates 698447..698629 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG574_RS03820 Protein ID WP_006250984.1
Coordinates 697989..698408 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG574_RS03775 693223..693657 - 435 WP_020824225.1 hypothetical protein -
BG574_RS03780 693751..694554 - 804 WP_020824224.1 DUF2303 family protein -
BG574_RS03785 694626..694985 - 360 WP_006250991.1 hypothetical protein -
BG574_RS03790 695021..695479 - 459 WP_006253113.1 single-stranded DNA-binding protein -
BG574_RS13030 695485..695649 - 165 WP_020824223.1 hypothetical protein -
BG574_RS03795 695633..695818 - 186 WP_020824222.1 hypothetical protein -
BG574_RS03800 696011..696334 + 324 WP_006250988.1 hypothetical protein -
BG574_RS03805 696813..697043 - 231 WP_006250987.1 hypothetical protein -
BG574_RS03810 697333..697608 + 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG574_RS03815 697617..697922 + 306 WP_006250985.1 HigA family addiction module antidote protein -
BG574_RS03820 697989..698408 - 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG574_RS03825 698447..698629 - 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG574_RS03830 698760..699245 - 486 WP_006252741.1 hypothetical protein -
BG574_RS03835 699250..699630 - 381 WP_006248823.1 hypothetical protein -
BG574_RS03840 699705..700388 - 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG574_RS03845 700519..700719 + 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG574_RS03850 700740..701000 + 261 WP_006252483.1 hypothetical protein -
BG574_RS03855 701176..702015 + 840 WP_006252350.1 Rha family transcriptional regulator -
BG574_RS03860 702012..703058 + 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 687641..769297 81656


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67091 WP_006250075.1 NZ_CP017509:c698629-698447 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67091 NZ_CP017509:c698629-698447 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67091 WP_006250984.1 NZ_CP017509:c698408-697989 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67091 NZ_CP017509:c698408-697989 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References