Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 378581..379229 Replicon chromosome
Accession NZ_CP017509
Organism Mannheimia haemolytica strain 28226

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG574_RS02070 Protein ID WP_006251221.1
Coordinates 379053..379229 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG574_RS02065 Protein ID WP_006251222.1
Coordinates 378581..378997 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG574_RS02025 374937..375371 + 435 WP_006251230.1 hypothetical protein -
BG574_RS02030 375386..376375 + 990 WP_006251229.1 encapsulin -
BG574_RS02035 376387..376584 + 198 WP_032848903.1 hypothetical protein -
BG574_RS02040 376587..376964 + 378 WP_006251227.1 hypothetical protein -
BG574_RS02045 376964..377308 + 345 WP_006251226.1 hypothetical protein -
BG574_RS02050 377310..377678 + 369 WP_061888643.1 hypothetical protein -
BG574_RS02055 377675..378055 + 381 WP_006251224.1 hypothetical protein -
BG574_RS02060 378059..378541 + 483 WP_006251223.1 phage tail protein -
BG574_RS02065 378581..378997 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG574_RS02070 379053..379229 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG574_RS02075 379325..379996 + 672 WP_006251220.1 hypothetical protein -
BG574_RS02080 380011..380280 - 270 WP_006251219.1 hypothetical protein -
BG574_RS02085 380413..380871 + 459 WP_006251218.1 hypothetical protein -
BG574_RS02090 380939..381334 + 396 WP_006251217.1 hypothetical protein -
BG574_RS02095 381421..383916 + 2496 WP_061888644.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 345927..392902 46975


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67089 WP_006251221.1 NZ_CP017509:c379229-379053 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67089 NZ_CP017509:c379229-379053 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67089 WP_006251222.1 NZ_CP017509:c378997-378581 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67089 NZ_CP017509:c378997-378581 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References