Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1957632..1958272 Replicon chromosome
Accession NZ_CP017508
Organism Mannheimia haemolytica strain 28240

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG565_RS09730 Protein ID WP_006250075.1
Coordinates 1957632..1957814 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG565_RS09735 Protein ID WP_006250984.1
Coordinates 1957853..1958272 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG565_RS09695 1953203..1954249 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG565_RS09700 1954246..1955085 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG565_RS09705 1955261..1955521 - 261 WP_006252483.1 hypothetical protein -
BG565_RS09710 1955542..1955742 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG565_RS09715 1955873..1956556 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG565_RS09720 1956631..1957011 + 381 WP_006248823.1 hypothetical protein -
BG565_RS09725 1957016..1957501 + 486 WP_006252741.1 hypothetical protein -
BG565_RS09730 1957632..1957814 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG565_RS09735 1957853..1958272 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG565_RS09740 1958339..1958644 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG565_RS09745 1958653..1958928 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG565_RS09750 1959218..1959448 + 231 WP_006250987.1 hypothetical protein -
BG565_RS09755 1959927..1960250 - 324 WP_006250988.1 hypothetical protein -
BG565_RS09760 1960443..1960628 + 186 WP_020824222.1 hypothetical protein -
BG565_RS13380 1960612..1960776 + 165 WP_020824223.1 hypothetical protein -
BG565_RS09765 1960782..1961240 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG565_RS09770 1961276..1961635 + 360 WP_006250991.1 hypothetical protein -
BG565_RS09775 1961707..1962510 + 804 WP_020824224.1 DUF2303 family protein -
BG565_RS09780 1962562..1962996 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1921276..1968081 46805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67084 WP_006250075.1 NZ_CP017508:1957632-1957814 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67084 NZ_CP017508:1957632-1957814 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67084 WP_006250984.1 NZ_CP017508:1957853-1958272 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67084 NZ_CP017508:1957853-1958272 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References