Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2298282..2298930 Replicon chromosome
Accession NZ_CP017507
Organism Mannheimia haemolytica strain 28253

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG566_RS11615 Protein ID WP_006251221.1
Coordinates 2298282..2298458 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG566_RS11620 Protein ID WP_006251222.1
Coordinates 2298514..2298930 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG566_RS11590 2293594..2296089 - 2496 WP_061888644.1 phage tail length tape measure family protein -
BG566_RS11595 2296176..2296571 - 396 WP_006251217.1 hypothetical protein -
BG566_RS11600 2296639..2297097 - 459 WP_006251218.1 hypothetical protein -
BG566_RS11605 2297266..2297499 + 234 WP_020824316.1 hypothetical protein -
BG566_RS11610 2297514..2298185 - 672 WP_006251220.1 hypothetical protein -
BG566_RS11615 2298282..2298458 + 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG566_RS11620 2298514..2298930 + 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG566_RS11625 2298970..2299452 - 483 WP_006251223.1 phage tail protein -
BG566_RS11630 2299456..2299836 - 381 WP_006251224.1 hypothetical protein -
BG566_RS11635 2299833..2300201 - 369 WP_006251225.1 hypothetical protein -
BG566_RS11640 2300203..2300547 - 345 WP_006251226.1 hypothetical protein -
BG566_RS11645 2300547..2300924 - 378 WP_006251227.1 hypothetical protein -
BG566_RS11650 2300927..2301223 - 297 WP_020828758.1 hypothetical protein -
BG566_RS11655 2301235..2302221 - 987 WP_062627922.1 encapsulin -
BG566_RS11660 2302236..2302670 - 435 WP_006251230.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2284608..2336286 51678


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67078 WP_006251221.1 NZ_CP017507:2298282-2298458 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67078 NZ_CP017507:2298282-2298458 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67078 WP_006251222.1 NZ_CP017507:2298514-2298930 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67078 NZ_CP017507:2298514-2298930 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References