Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1957609..1958249 Replicon chromosome
Accession NZ_CP017507
Organism Mannheimia haemolytica strain 28253

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG566_RS09745 Protein ID WP_006250075.1
Coordinates 1957609..1957791 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG566_RS09750 Protein ID WP_006250984.1
Coordinates 1957830..1958249 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG566_RS09710 1953180..1954226 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG566_RS09715 1954223..1955062 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG566_RS09720 1955238..1955498 - 261 WP_006252483.1 hypothetical protein -
BG566_RS09725 1955519..1955719 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG566_RS09730 1955850..1956533 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG566_RS09735 1956608..1956988 + 381 WP_006248823.1 hypothetical protein -
BG566_RS09740 1956993..1957478 + 486 WP_006252741.1 hypothetical protein -
BG566_RS09745 1957609..1957791 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG566_RS09750 1957830..1958249 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG566_RS09755 1958316..1958621 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG566_RS09760 1958630..1958905 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG566_RS09765 1959195..1959425 + 231 WP_006250987.1 hypothetical protein -
BG566_RS09770 1959904..1960227 - 324 WP_006250988.1 hypothetical protein -
BG566_RS09775 1960420..1960605 + 186 WP_020824222.1 hypothetical protein -
BG566_RS13375 1960589..1960753 + 165 WP_020824223.1 hypothetical protein -
BG566_RS09780 1960759..1961217 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG566_RS09785 1961253..1961612 + 360 WP_006250991.1 hypothetical protein -
BG566_RS09790 1961684..1962487 + 804 WP_020824224.1 DUF2303 family protein -
BG566_RS09795 1962539..1962973 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1921253..1968058 46805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67076 WP_006250075.1 NZ_CP017507:1957609-1957791 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67076 NZ_CP017507:1957609-1957791 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67076 WP_006250984.1 NZ_CP017507:1957830-1958249 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67076 NZ_CP017507:1957830-1958249 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References