Detailed information of TA system TA067068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1645868..1646508
• Replicon: chromosome
• Accession: NZ_CP017506
• Organism: Mannheimia haemolytica strain 28488

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG601_RS08485
• Protein ID: WP_006250075.1
• Coordinates: 1645868..1646050 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG601_RS08490
• Protein ID: WP_006250074.1
• Coordinates: 1646089..1646508 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG601_RS08440	1641415..1641708	-	294	WP_006247812.1	hypothetical protein	-
BG601_RS08445	1641852..1642241	+	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG601_RS08450	1642213..1642512	+	300	WP_006247810.1	XRE family transcriptional regulator	-
BG601_RS14255	1642521..1642679	-	159	WP_006247809.1	hypothetical protein	-
BG601_RS14260	1642689..1643756	-	1068	WP_006253284.1	hypothetical protein	-
BG601_RS08465	1643777..1643977	-	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG601_RS08470	1644108..1644791	+	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG601_RS08475	1644866..1645246	+	381	WP_006248823.1	hypothetical protein	-
BG601_RS08480	1645251..1645736	+	486	WP_015484250.1	hypothetical protein	-
BG601_RS08485	1645868..1646050	+	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG601_RS08490	1646089..1646508	+	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG601_RS08495	1646844..1647488	+	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG601_RS08505	1647626..1648666	+	1041	WP_015587044.1	IS481 family transposase	-
BG601_RS08510	1648733..1650139	-	1407	WP_006253513.1	YdgA family protein	-
BG601_RS08515	1650189..1650995	-	807	WP_006248821.1	tryptophan synthase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1581995..1650139	68144
	-	flank	IS/Tn	-	-	1647626..1648666	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67068 WP_006250075.1 NZ_CP017506:1645868-1646050 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67068 NZ_CP017506:1645868-1646050 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67068 WP_006250074.1 NZ_CP017506:1646089-1646508 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67068 NZ_CP017506:1646089-1646508 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9