Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1870737..1871276 Replicon chromosome
Accession NZ_CP017504
Organism Mannheimia haemolytica strain 32635

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG584_RS09670 Protein ID WP_006247804.1
Coordinates 1871007..1871276 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG584_RS09665 Protein ID WP_006247803.1
Coordinates 1870737..1871003 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG584_RS09610 1866137..1866772 - 636 Protein_1867 transposase -
BG584_RS09620 1866873..1867583 - 711 Protein_1868 tyrosine-type recombinase/integrase -
BG584_RS09625 1867487..1867810 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG584_RS09630 1867891..1868058 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG584_RS09635 1868066..1868440 - 375 WP_006247798.1 hypothetical protein -
BG584_RS09640 1868590..1868817 + 228 Protein_1872 phage terminase large subunit family protein -
BG584_RS09645 1868842..1869237 + 396 WP_006247799.1 phage tail protein -
BG584_RS09650 1869269..1869910 + 642 WP_006247800.1 hypothetical protein -
BG584_RS09655 1869993..1870394 + 402 WP_006247801.1 hypothetical protein -
BG584_RS09660 1870439..1870669 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG584_RS09665 1870737..1871003 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG584_RS09670 1871007..1871276 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG584_RS09675 1871351..1871614 + 264 WP_006247805.1 hypothetical protein -
BG584_RS09680 1871674..1872384 + 711 WP_006253312.1 tape measure protein -
BG584_RS09685 1872362..1872652 + 291 Protein_1881 C40 family peptidase -
BG584_RS09690 1872642..1873361 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG584_RS09695 1873583..1874209 + 627 WP_006247807.1 tail assembly protein -
BG584_RS09700 1874258..1874869 + 612 WP_006253310.1 hypothetical protein -
BG584_RS09705 1874980..1875666 + 687 WP_006247808.1 hypothetical protein -
BG584_RS14040 1875676..1875834 + 159 WP_006247809.1 hypothetical protein -
BG584_RS09710 1875843..1876142 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1856830..1878617 21787
- flank IS/Tn - - 1866080..1866772 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67055 WP_006247804.1 NZ_CP017504:1871007-1871276 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67055 NZ_CP017504:1871007-1871276 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67055 WP_006247803.1 NZ_CP017504:1870737-1871003 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67055 NZ_CP017504:1870737-1871003 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References