Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1180530..1181178 Replicon chromosome
Accession NZ_CP017504
Organism Mannheimia haemolytica strain 32635

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG584_RS06190 Protein ID WP_006251221.1
Coordinates 1181002..1181178 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG584_RS06185 Protein ID WP_006251222.1
Coordinates 1180530..1180946 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG584_RS06145 1176823..1177257 + 435 WP_006251230.1 hypothetical protein -
BG584_RS06150 1177272..1178261 + 990 WP_006251229.1 encapsulin -
BG584_RS06155 1178273..1178533 + 261 WP_020824317.1 hypothetical protein -
BG584_RS06160 1178536..1178913 + 378 WP_006251227.1 hypothetical protein -
BG584_RS06165 1178913..1179257 + 345 WP_006251226.1 hypothetical protein -
BG584_RS06170 1179259..1179627 + 369 WP_006251225.1 hypothetical protein -
BG584_RS06175 1179624..1180004 + 381 WP_006251224.1 hypothetical protein -
BG584_RS06180 1180008..1180490 + 483 WP_006251223.1 phage tail protein -
BG584_RS06185 1180530..1180946 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG584_RS06190 1181002..1181178 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG584_RS06195 1181274..1181945 + 672 WP_006251220.1 hypothetical protein -
BG584_RS06200 1181960..1182193 - 234 WP_020824316.1 hypothetical protein -
BG584_RS06205 1182362..1182820 + 459 WP_006251218.1 hypothetical protein -
BG584_RS06215 1183365..1185860 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1074674..1197853 123179


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67054 WP_006251221.1 NZ_CP017504:c1181178-1181002 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67054 NZ_CP017504:c1181178-1181002 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67054 WP_006251222.1 NZ_CP017504:c1180946-1180530 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67054 NZ_CP017504:c1180946-1180530 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References