Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1818240..1818779 Replicon chromosome
Accession NZ_CP017501
Organism Mannheimia haemolytica strain 33041

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG597_RS09360 Protein ID WP_006247804.1
Coordinates 1818510..1818779 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG597_RS09355 Protein ID WP_006247803.1
Coordinates 1818240..1818506 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG597_RS09300 1813640..1814275 - 636 Protein_1803 transposase -
BG597_RS09310 1814376..1815086 - 711 Protein_1804 tyrosine-type recombinase/integrase -
BG597_RS09315 1814990..1815313 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG597_RS09320 1815394..1815561 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG597_RS09325 1815569..1815943 - 375 WP_006247798.1 hypothetical protein -
BG597_RS09330 1816093..1816320 + 228 Protein_1808 phage terminase large subunit family protein -
BG597_RS09335 1816345..1816740 + 396 WP_006247799.1 phage tail protein -
BG597_RS09340 1816772..1817413 + 642 WP_006247800.1 hypothetical protein -
BG597_RS09345 1817496..1817897 + 402 WP_006247801.1 hypothetical protein -
BG597_RS09350 1817942..1818172 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG597_RS09355 1818240..1818506 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG597_RS09360 1818510..1818779 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG597_RS09365 1818854..1819117 + 264 WP_006247805.1 hypothetical protein -
BG597_RS09370 1819177..1819887 + 711 WP_006253312.1 tape measure protein -
BG597_RS09375 1819865..1820155 + 291 Protein_1817 C40 family peptidase -
BG597_RS09380 1820145..1820864 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG597_RS09385 1821086..1821712 + 627 WP_006247807.1 tail assembly protein -
BG597_RS09390 1821761..1822372 + 612 WP_006253310.1 hypothetical protein -
BG597_RS09395 1822483..1823169 + 687 WP_006247808.1 hypothetical protein -
BG597_RS13700 1823179..1823337 + 159 WP_006247809.1 hypothetical protein -
BG597_RS09400 1823346..1823645 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1804333..1826120 21787
- flank IS/Tn - - 1813583..1814275 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67034 WP_006247804.1 NZ_CP017501:1818510-1818779 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67034 NZ_CP017501:1818510-1818779 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67034 WP_006247803.1 NZ_CP017501:1818240-1818506 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67034 NZ_CP017501:1818240-1818506 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References