Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2333644..2334233 Replicon chromosome
Accession NZ_CP017500
Organism Mannheimia haemolytica strain 33690

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag BG562_RS11865 Protein ID WP_006248516.1
Coordinates 2333901..2334233 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag BG562_RS11860 Protein ID WP_006248517.1
Coordinates 2333644..2333904 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG562_RS11835 2328823..2329968 - 1146 WP_006251102.1 FMN-dependent L-lactate dehydrogenase LldD -
BG562_RS11840 2330580..2331224 + 645 WP_006248521.1 RNA pyrophosphohydrolase -
BG562_RS11845 2331226..2332014 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
BG562_RS11850 2332022..2332819 + 798 WP_006251103.1 prolipoprotein diacylglyceryl transferase -
BG562_RS11855 2332906..2333574 + 669 WP_006248518.1 phosphoglycolate phosphatase -
BG562_RS11860 2333644..2333904 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG562_RS11865 2333901..2334233 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BG562_RS11870 2334360..2335391 + 1032 WP_006248514.1 tryptophan--tRNA ligase -
BG562_RS11875 2335455..2336210 - 756 WP_006248513.1 M48 family metallopeptidase -
BG562_RS11880 2336266..2336793 - 528 WP_006248512.1 inorganic diphosphatase -
BG562_RS11900 2337373..2338407 + 1035 WP_006248509.1 endolytic transglycosylase MltG -
BG562_RS11905 2338418..2339038 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T67028 WP_006248516.1 NZ_CP017500:2333901-2334233 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T67028 NZ_CP017500:2333901-2334233 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT67028 WP_006248517.1 NZ_CP017500:2333644-2333904 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT67028 NZ_CP017500:2333644-2333904 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References