Detailed information of TA system TA067027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2306321..2306969
• Replicon: chromosome
• Accession: NZ_CP017500
• Organism: Mannheimia haemolytica strain 33690

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG562_RS11635
• Protein ID: WP_006251221.1
• Coordinates: 2306321..2306497 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG562_RS11640
• Protein ID: WP_006251222.1
• Coordinates: 2306553..2306969 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG562_RS11610	2301634..2304129	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG562_RS11615	2304216..2304611	-	396	WP_006251217.1	hypothetical protein	-
BG562_RS11620	2304679..2305137	-	459	WP_006251218.1	hypothetical protein	-
BG562_RS11625	2305306..2305539	+	234	WP_020824316.1	hypothetical protein	-
BG562_RS11630	2305554..2306225	-	672	WP_006251220.1	hypothetical protein	-
BG562_RS11635	2306321..2306497	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG562_RS11640	2306553..2306969	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG562_RS11645	2307009..2307491	-	483	WP_006251223.1	phage tail protein	-
BG562_RS11650	2307495..2307875	-	381	WP_006251224.1	hypothetical protein	-
BG562_RS11655	2307872..2308240	-	369	WP_061888643.1	hypothetical protein	-
BG562_RS11660	2308242..2308586	-	345	WP_006251226.1	hypothetical protein	-
BG562_RS11665	2308586..2308963	-	378	WP_006251227.1	hypothetical protein	-
BG562_RS11670	2308966..2309163	-	198	WP_032848903.1	hypothetical protein	-
BG562_RS11675	2309175..2310164	-	990	WP_006251229.1	encapsulin	-
BG562_RS11680	2310179..2310613	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2292648..2328769	36121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67027 WP_006251221.1 NZ_CP017500:2306321-2306497 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67027 NZ_CP017500:2306321-2306497 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67027 WP_006251222.1 NZ_CP017500:2306553-2306969 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67027 NZ_CP017500:2306553-2306969 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6