Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1966819..1967459 Replicon chromosome
Accession NZ_CP017500
Organism Mannheimia haemolytica strain 33690

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG562_RS09770 Protein ID WP_006250075.1
Coordinates 1966819..1967001 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG562_RS09775 Protein ID WP_006250984.1
Coordinates 1967040..1967459 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG562_RS09735 1962390..1963436 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG562_RS09740 1963433..1964272 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG562_RS09745 1964448..1964708 - 261 WP_006252483.1 hypothetical protein -
BG562_RS09750 1964729..1964929 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG562_RS09755 1965060..1965743 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG562_RS09760 1965818..1966198 + 381 WP_006248823.1 hypothetical protein -
BG562_RS09765 1966203..1966688 + 486 WP_006252741.1 hypothetical protein -
BG562_RS09770 1966819..1967001 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG562_RS09775 1967040..1967459 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG562_RS09780 1967526..1967831 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG562_RS09785 1967840..1968115 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG562_RS09790 1968405..1968635 + 231 WP_006250987.1 hypothetical protein -
BG562_RS09795 1969114..1969437 - 324 WP_006250988.1 hypothetical protein -
BG562_RS09800 1969630..1969815 + 186 WP_020824222.1 hypothetical protein -
BG562_RS13300 1969799..1969963 + 165 WP_020824223.1 hypothetical protein -
BG562_RS09805 1969969..1970427 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG562_RS09810 1970463..1970822 + 360 WP_006250991.1 hypothetical protein -
BG562_RS09815 1970894..1971697 + 804 WP_020824224.1 DUF2303 family protein -
BG562_RS09820 1971791..1972225 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1924259..1977310 53051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67025 WP_006250075.1 NZ_CP017500:1966819-1967001 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67025 NZ_CP017500:1966819-1967001 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67025 WP_006250984.1 NZ_CP017500:1967040-1967459 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67025 NZ_CP017500:1967040-1967459 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References