Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 491062..491712 Replicon chromosome
Accession NZ_CP017500
Organism Mannheimia haemolytica strain 33690

Toxin (Protein)


Gene name vapC Uniprot ID A0A378NBZ4
Locus tag BG562_RS02435 Protein ID WP_020831127.1
Coordinates 491323..491712 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D2Q6P1
Locus tag BG562_RS02430 Protein ID WP_006251766.1
Coordinates 491062..491322 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG562_RS02415 486750..490283 + 3534 WP_006251763.1 transcription-repair coupling factor -
BG562_RS02420 490446..490733 + 288 WP_020831126.1 BrnT family toxin -
BG562_RS02425 490734..490946 + 213 WP_006251765.1 BrnA antitoxin family protein -
BG562_RS02430 491062..491322 + 261 WP_006251766.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG562_RS02435 491323..491712 + 390 WP_020831127.1 type II toxin-antitoxin system VapC family toxin Toxin
BG562_RS02440 491732..492970 + 1239 WP_006251767.1 peptidase T -
BG562_RS02475 493832..494617 + 786 WP_006251768.1 hypothetical protein -
BG562_RS02480 494691..495551 + 861 WP_006250124.1 metal ABC transporter permease -
BG562_RS02485 495544..496353 + 810 WP_006250123.1 metal ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14575.87 Da        Isoelectric Point: 7.7391

>T67023 WP_020831127.1 NZ_CP017500:491323-491712 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLGYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T67023 NZ_CP017500:491323-491712 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGGTTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.50 Da        Isoelectric Point: 4.6909

>AT67023 WP_006251766.1 NZ_CP017500:491062-491322 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT67023 NZ_CP017500:491062-491322 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NBZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D2Q6P1

References